Essays about: "preparative chromatography"

Showing result 1 - 5 of 7 essays containing the words preparative chromatography.

  1. 1. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

    University essay from Karlstads universitet

    Author : Rebecka Jasinski; [2021]
    Keywords : oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

    Abstract : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. READ MORE

  2. 2. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    University essay from Karlstads universitet

    Author : Lewis Frazer; [2021]
    Keywords : Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Abstract : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. READ MORE

  3. 3. Iterative Learning Control for Preparative Chromatography

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Daniel Espinoza; [2020]
    Keywords : Chemical Engineering; Kemiteknik; Chromatography; Automatic Process Control; Iterative Learning Control; ILC; Simulation; Protein Purification; Python; Ion-Exchange; Automation; Batch Processing; Downstream Processing; Technology and Engineering;

    Abstract : There is a desire for a fully automated downstream process in pharmaceutical protein production. One part of the downstreaming process is preparative chromatography. A good separation between the product and other proteins, as well as good productivity, are desired. READ MORE

  4. 4. Automated Simulation and Parameter Estimation of Chromatography Processes

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Johan Olsson; [2019]
    Keywords : chemical engineering; kemiteknik; Technology and Engineering;

    Abstract : Preparative chromatography is an important separation technique in downstream processing for several industries. It has the ability to separate two or more component from each other to achieve high purity. READ MORE

  5. 5. Chromatography of Therapeutic Peptides - Contrasting SFC and HPLC

    University essay from Uppsala universitet/Farmakognosi

    Author : Joakim Bagge; [2019]
    Keywords : ;

    Abstract : This work is a comparison of a well-established and a novel, "green" and efficient technique to separate peptides of pharmaceutical interest. An attempt is made to derive the chromatographic retention behaviour from these techniques to a number of property descriptors derived from the linear sequence of amino acids. READ MORE