Essays about: "primers"

Showing result 1 - 5 of 76 essays containing the word primers.

  1. 1. Development of a novel quantitative PCR analysis method for HIV-1

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Author : Anna-Malin Hallberg; [2021]
    Keywords : HIV; PCR; primers; probes; virus; detection; blood plasma; pathogen; biological pharmaceutical; protein-based pharmaceutical; genotype; subtype; RT qPCR; extraction; TaqMa; RNA virus; cross-dimer; applied biochemistry; tillämpad biokemi; Biology and Life Sciences; Technology and Engineering; Chemistry;

    Abstract : Biological pharmaceuticals are the answer to many severe diseases of today’s society. Some of these are protein-based drugs and can be produced from human blood plasma. In order to guarantee the safety of patients, absence of any pathogens needs to be ensured. READ MORE

  2. 2. Jämförelse mellan okulär och molekylär diagnostisering av stråbassjukdomar i höstvete

    University essay from SLU/Dept. of Forest Mycology and Plant Pathology

    Author : Agnes Radovic; [2020]
    Keywords : Oculimacula yallundae; Oculimacula acuformis; Fusarium spp; Rhizictonia cerealis; PCR; stråbassjukdomar;

    Abstract : I dagens jordbruk baseras diagnoser och rekommendationer kring kemisk be-kämpning till stor del på okulär diagnostisering gjord i fält. Den okulära diagnostise-ringen är ett bra verktyg, men i vissa fall är det svårt att se skillnad på liknande symtom orsakade av olika patogener. READ MORE

  3. 3. Development and validation of a novel quantitative PCR analysis method for HIV-1

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Author : Josefine Sundgren; [2020]
    Keywords : HIV-1; qPCR; Octapharma; Taqman; applied biochemistry; tillämpad biokemi; Technology and Engineering;

    Abstract : The major problem when developing primers and probe sets for detection of HIV-1 is its high genetic variability and strong ability to mutate. HIV-1 is divided into four groups, M, N, O, P based on genetic similarities, and there are more than 70 different circulating recombinant forms. READ MORE

  4. 4. Optimization of the Two-Tailed RT qPCR method with synthetic miR-16 and miR-210 in human plasma for future diagnostics of sepsis

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Michelle Von Ehr; [2020]
    Keywords : ;

    Abstract : Every year, around 6 million people lose the fight against sepsis. Sepsis is a dysregulated response from the host to an infection, leading to life-threatening organ dysfunction. READ MORE

  5. 5. Optimization of a Multiplex PCR-RFLP Method Used for Detection of Three Primary Mutations in Leber’s Hereditary Optic Neuropathy Patients

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Emilia Nord; [2020]
    Keywords : LHON; Restriction enzyme; Multiplex PCR; Mutation detection; Mitochondrial mutation;

    Abstract : Leber’s hereditary optic neuropathy (LHON) is the most commonly inherited disease that causes blindness in one or both eyes, with a minimum prevalence of 1 in 31 000 in the northeast of England. What causes LHON is not fully known but three mitochondrial mutations, G3460A, G11778A, and T14484C, have been identified in over 95 % of all LHON patients. READ MORE