Essays about: "protein denaturation"

Showing result 1 - 5 of 14 essays containing the words protein denaturation.

  1. 1. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

    University essay from Linköpings universitet/Kemi

    Author : Anton Elfstrand; [2023]
    Keywords : Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

    Abstract : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. READ MORE

  3. 2. Predicting Protein Stability with Machine Learning

    University essay from Lunds universitet/Biofysikalisk kemi

    Author : Lucas Carlsson; [2023]
    Keywords : Protein Stability; Biophysical Chemistry; Thermodynamics; Machine Learning; Deep Learning; Protein Language Model; Biochemistry; Multiple Sequence Alignment; Bioinformatics; Biology and Life Sciences;

    Abstract : Protein stability is a property of high importance and is of interest in a variety of fields. It determines if a protein has its native fold and can be of influence in certain diseases such as Parkinson's and Alzheimer's disease. READ MORE

  4. 3. Phenol-Protein Solution Stability

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master)

    Author : Johan Sjölund; [2022]
    Keywords : Phenol; Protein; Proteins; Lysozyme; Betalactoglobulin; Trastuzumab; Lipase; GAZ; GA-Z; Palifermin; Titration; Probedrum; Pharmaceutical Technology; Chemistry;

    Abstract : Phenol is a commonly used preservative in pharmaceutical protein solutions. It is compatible with most proteins but can cause aggregation and denaturation at higher concentrations or in more sensitive proteins. READ MORE

  5. 4. Alkaline Protein Extraction of Oat Bran and Oat Endosperm Flour - the Effect of pH during Extraction and Precipitation

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master)

    Author : Bella Nielsen; Alva Rodhe; [2021]
    Keywords : Oats; Protein; Alkaline extraction; Protein extraction; Plant-based proteins; Oat protein; Oat bran; Oat endosperm; Iso-electric precipitation; Food engineering; Technology and Engineering; Agriculture and Food Sciences;

    Abstract : An increased understanding of the negative impacts of animal-based proteins, on both environment and health, has led to a rise in the demand for plant-based proteins. To meet the demands, conventional crops must be efficiently utilized. READ MORE

  6. 5. Investigation of possible improvements of the stability of bispecific ADAPT proteins

    University essay from KTH/Proteinvetenskap

    Author : Ella Eriksson; [2021]
    Keywords : Protein purification; Affinity chromatography; ABD-derived domain; ADAPT protein; simultan binding;

    Abstract : The aim of the project was to identify positions and amino acids that contribute to improved structure and stability of bispecific ADAPT proteins. During the 20 weeks project period, different amino acid substitutions were analysed to evaluate the effect on the three-helical structure and stability of bispecific ADAPTs targeting human serum albumin (HSA) and tumor necrosis factor α (TNFα). READ MORE