Essays about: "reagent optimization"

Showing result 1 - 5 of 9 essays containing the words reagent optimization.

  1. 1. An investigation of o-phthaldialdehyde as a derivatization reagent and optimization of instrumental parameters for qualitative amino acid profiling via UPLC-UV

    University essay from Uppsala universitet/Institutionen för kemi - BMC

    Author : Annie Aspö; [2023]
    Keywords : ;

    Abstract : .... READ MORE

  3. 2. Investigations of miR-34a structure and dynamics by SHAPE MaP and optimal time between transfection and modification by qPCR

    University essay from KTH/Proteinvetenskap

    Author : Ellinor Lindén; [2023]
    Keywords : SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a; SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a;

    Abstract : microRNA (miRNA) är korta icke-kodande RNA sekvenser som har fått ökande uppmärksamhet då de kan binda till mRNA och styra vårt genuttryck. Omkring trettio procent av våra proteinkodande gener styrs av miRNA. miRNA binder till mRNA vilket hindrar dess translation eller leder till nedbrytning av mRNA. READ MORE

  4. 3. A study of the adsorption isotherms of Ion-pair reagents

    University essay from Karlstads universitet

    Author : Mehmet Bilici; [2022]
    Keywords : Tributylamine; adsorption isotherm; liquid-liquid extraction; gas chromatography;

    Abstract : In this project, the optimization of obtaining adsorption isotherms for the ion pair reagent, tributylamine was tested. The goal was to have a better understanding of the chromatographic process when separating biomolecules, such as oligonucleotides. READ MORE

  5. 4. Characterization of NeuN expression in the mouse neuronal NSC-34 cell line using RT-qPCR, immunological staining and siRNA-mediated gene suppression

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Henrik Hallgren; [2019]
    Keywords : Rbfox-3; Neuronal Nucleus; motor neuron; differentiation; secondary injury;

    Abstract : Background: Acute spinal trauma is followed by a secondary injury that causes additional damage to the tissue. The mouse neuronal hybrid cell line NSC-34 is planned for studies regarding this process, wherefore the cell line needed to be established in the laboratory and a proof-of-concept study needed to be performed. READ MORE

  6. 5. Optimization of a pharmacokinetic assay in a bridging assay format using the Gyrolab immunoassay platform

    University essay from Uppsala universitet/Biokemi

    Author : Ebba Spetsare; [2019]
    Keywords : bridging assay; gyros; affinity; humira; tnf-alfa; pharmacokinetic;

    Abstract : Anti-TNF alpha antibodies were among the first approved antibody drugs and now belongs to the best-selling drugs. Today, several companies are developing biosimilars to those drugs which will increase the access of medications and potentially reduce health care costs. READ MORE