Essays about: "recombinant E.coli"

Showing result 1 - 5 of 31 essays containing the words recombinant E.coli.

  1. 1. The production of recombinant 6LZE_A and NS2B-NS3 in E. coli

    University essay from Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Author : Raluca Svensson; [2024]
    Keywords : coronavirus; dengue; protease; pandemic; SARS-Cov-2; DENV; viral replication; proteins; Escherichia coli; DL-BAPNA; computational modeling; recombinant protein production; biotechnology; Biology and Life Sciences;

    Abstract : Even though the first coronavirus pandemic was registered in 1965, SARS-CoV-2 was better acknowledged when it caused the worldwide pandemic in 2019 in which millions of individuals lost their lives. Due to its facile way of spreading from one individual to another, the number of infected cases has gone up to more than 750 million to this day. READ MORE

  3. 2. Sortase A coupling of the recombinant partial silk proteins 4Rep-Srt and G-/G5-CT to understand the structure of silk fiber

    University essay from KTH/Proteinvetenskap

    Author : Ellen Adlerz; [2023]
    Keywords : 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A coupling; recombinant silk protein; 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A-koppling; rekombinant silkesprotein;

    Abstract : Spindelsilke är ett intressant material på grund av dess biokompatibilitet och därav användning som biomaterial. Det är jämförbart med konstgjorda material när det gäller styrka och elasticitet, och i kombination med biokompatibiliteten, är spindelsilke eftertraktat inom det medicinska området. READ MORE

  4. 3. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Pennie Lindblom; Olivia Geel; [2023]
    Keywords : Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Abstract : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. READ MORE

  5. 4. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    University essay from Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Author : Erik Apelqvist; [2022]
    Keywords : Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Abstract : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. READ MORE

  6. 5. Recombinant Expression of Novel Wild Type Oat Hemoglobins in Escherichia Coli

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Frank Rummukainen; [2022]
    Keywords : Recombinant technology; Protein expression; Hemoglobin; Phytoglobin; Escherichia coli; Oat; Avena sativa; Heme; Iron; Biosynthesis; Transcription; qPCR; SDS-PAGE; Fermentation; Recombination; Applied Biochemistry; Biology and Life Sciences;

    Abstract : Hemoglobin is a protein transporting oxygen through our bloodstreams and is as such essential for aerobic life. Hemoglobins also exist in plants, with a wide variety of functions other than oxygen transport. READ MORE