Essays about: "rolling circle amplification"

Showing result 1 - 5 of 18 essays containing the words rolling circle amplification.

  1. 1. A novel assay for the detection of Hepatitis C virus in blood plasma, using padlock probes and rolling circle amplification

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Andréa Karlsson; [2023]
    Keywords : ;

    Abstract : Hepatitis C viral infection is a globally widespread blood-borne disease affecting the liver, causing cirrhosis and hepatocellular carcinoma. This type of liver cancer is mainly caused by chronic Hepatitis C and Hepatitis B. Specific detection with the following correct treatment is crucial to reduce the overall burden of the disease. READ MORE

  3. 2. Quantification of DNA Nanoballs Using Image Processing Techniques

    University essay from Uppsala universitet/Avdelningen Vi3

    Author : Sara Lindberg; [2023]
    Keywords : image analysis; fluorescence microscopy; quantification; DNA; rolling circle amplification; machine learning; deep learning; transfer learning; neural networks;

    Abstract : In gene editing, it is important to identify the number of edited and unedited nucleic acids in the development of new therapies and drugs. Countagen is developing a technology for accelerating genomic research and their product is called GeneAbacus. READ MORE

  4. 3. Quantification of DNA Microballs Using Image Processing Techniques

    University essay from KTH/Industriell bioteknologi

    Author : Yosef Werede Tedros; [2023]
    Keywords : Spot detection; Deep learning; Image processing; smFISH; Gene editing; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot; Detektering av punkter; Djupinlärning; Bildbehandling; smFISH; Genredigering; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot;

    Abstract : I detta examensarbete användes olika bildbehandlingstekniker för detektion och kvantifiering av DNA-mikrobollar, mer specifikt rolling circle amplification-produkter, på mikroskopibilder. Avsikten med detta arbete var att hjälpa Countagen AB utforska pipelines för bildbehandling för sin produkt där de analyserar utfallet av genredigeringsförsök på ett billigare och snabbare sätt än dagens konventionella sekvenseringsmetoder. READ MORE

  5. 4. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries

    University essay from Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Nicole Eger; [2021]
    Keywords : Genotyping; Genomic barcodes; LAMP; PNA; RCA; FISH;

    Abstract : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. READ MORE

  6. 5. Circle-to-circle amplification to improve the sensitivity of a magnetic nanoparticle-based DNA detection protocol

    University essay from Uppsala universitet/Molekylärbiologi

    Author : Anna Nilsson; [2021]
    Keywords : circle-to-circle amplification; rolling circle amplification; nanotechnology; DNA detection; magnetic nanoparticles;

    Abstract : Magnetic nanoparticles have great potential in the biomedical and diagnostics field. Due to their small size, the particles have a high surface-to-volume ratio which enables for biofunctionalisation with different molecular probes. This makes itpossible to target them against a wide variety of biomarkers. READ MORE