Essays about: "single molecule fluorescence"

Showing result 1 - 5 of 13 essays containing the words single molecule fluorescence.

  1. 1. Developing Automated Cell Segmentation Models Intended for MERFISH Analysis of the Cardiac Tissue by Deploying Supervised Machine Learning Algorithms

    University essay from KTH/Kemi

    Author : Julia Rune; [2023]
    Keywords : Cell Segmentation; Cellpose; Supervised Machine Learning; MERFISH; Heart Failure; Cellsegmentering; Cellpose; Övervakad Maskininlärning; MERFISH; Hjärtsvikt;

    Abstract : Följande studie behandlar utvecklandet av automatiserade cellsegmenteringsmodeller med avsikt att identifiera gränser mellan celler i hjärtvävnad. Syftet är att möjliggöra analys av data genererad från multiplexed error-robust in situ hybridization (MERFISH). READ MORE

  3. 2. Study of Automated Step Detection Methods and Dwell Time Analysis of Single-Molecule Data : Case of Study: The Turnover of Single Fluorescent ATP on The Active Site of Myosin

    University essay from Linnéuniversitetet/Institutionen för fysik och elektroteknik (IFE)

    Author : Lathifa Nur Ramdhania; [2022]
    Keywords : Single-molecule; step detection; dwell time analysis;

    Abstract : Single-molecule methods were developed with the objective to obtain kinetic information of biomolecular processes from a single biomolecule. This objective can be achieved by analyzing the binding events in recordings (“trajectories”) of single molecules (e.g., using fluorescence); the so-called dwell times. READ MORE

  4. 3. Assessment of promoter regulation in Pseudomonas putida using GFP and flow cytometry

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Luka Cululejevic; [2022]
    Keywords : applied microbiology; Pseudomonas putida; promoter characterization; aromatic compounds; vanillin; guaiacol; Biology and Life Sciences;

    Abstract : Lignin is found in all biomass and is an abundant natural source of aromatic compounds. It has the potential to serve as a renewable raw material for a variety of chemicals currently derived from fossil fuel sources such as muconic acid, an important platform chemical. Kraft lignin is a waste stream from the pulp and paper industry. READ MORE

  5. 4. Polarisation-resolved super-resolution microscopy

    University essay from Lunds universitet/Fasta tillståndets fysik; Lunds universitet/Fysiska institutionen

    Author : Wouter Duverger; [2021]
    Keywords : biophysics; microscopy; super-resolution microscopy; polarisation microscopy; STED; Physics and Astronomy;

    Abstract : This project aims to implement polarisation microscopy on the Tegenfeldt STED (stimulated emission depletion) microscope. STED microscopy is a targeted optical super-resolution method that can attain sub-diffraction resolution using visible light. READ MORE

  6. 5. Improving the temporal resolution of a microspectrometer for the study of the photophysics of enhanced green fluorescent protein

    University essay from KTH/Tillämpad fysik

    Author : Lukas Rane; [2021]
    Keywords : Biophysics; EGFP; Fluorescence; triplet state; Biofysik; EGFP; fluorescens; triplet tillstånd;

    Abstract : The use of fluorescent proteins as fluorescent markers has exploded over the last decades. In particular due to the development of advanced microscopy for live cell measurements, dynamic molecular studies down to single molecule levels and for superresolution microscopy. READ MORE