Essays about: "strukturbestämning"

Showing result 1 - 5 of 9 essays containing the word strukturbestämning.

  1. 1. Effects of Ultrafast Ionization in X-ray Coherent Diffraction Imaging

    University essay from KTH/Kemi

    Author : Arezu Sehati; [2023]
    Keywords : Coherent Diffraction Imaging; X-ray Free Electron Lasers; Refractive index; Core-shell nanoclusters; Rare-gas ionization; Koherent Diffraktionsavbildning; Röntgenfrielektronlaser; Brytningsindex; Core-shell-nanokluster; Ädelgasjonisering;

    Abstract : Koherent Diffraktionsavbildning med en intensiv röntgenpuls från en Frielektronlaser har möjliggjort strukturbestämning av isolerade nanopartiklar som inte går att studera med hjälp av konventionella metoder, så som elektronmikroskopi. En mycket kort pulslängd tillsammans med spridningssignalens beroende av objektets elektroniska struktur medför också möjligheten att studera inducerad ultrasnabb dynamik med denna teknik. READ MORE

  3. 2. Engineered imaging scaffolds for cryo-EM of small proteins of interest

    University essay from KTH/Proteinvetenskap

    Author : Oscar Friberg; [2022]
    Keywords : Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

    Abstract : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. READ MORE

  4. 3. Overexpression and purification of MSP2N2 for nanodisc assembly and reconstitution of TRPA1

    University essay from Lunds universitet/Examensarbeten i molekylärbiologi

    Author : Isabelle Bäckström; [2021]
    Keywords : Biology and Life Sciences;

    Abstract : Membrane proteins are important building blocks for several biochemical processes. The difficulty with membrane proteins is that they tend to be lowly expressed and can be difficult to manage. Therefore, membrane proteins are studied to a lesser extent than soluble proteins. READ MORE

  5. 4. Purification Methods for Solid-State NMR Analysis of Influenza A M2[18-60]

    University essay from Lunds universitet/Biofysikalisk kemi

    Author : Maxine Gripberg; [2021]
    Keywords : Solid-state NMR; Influenza A; M2 proton channel; Biophysical chemistry; Biology and Life Sciences; Technology and Engineering; Chemistry;

    Abstract : With viral infections becoming an ever-increasing health problem in the world, the study of viral proteins is a cornerstone for understanding and preventing future pandemics. The following work shows the purification and structural characterization of the Influenza A M2 proton channel through solid-state NMR (ssNMR). READ MORE

  6. 5. Design, production and evaluation of cross linked target proteins to an affibody-based carrier framework aimed for affinity protein: antigen structure determination using single particle Cryo-EM

    University essay from KTH/Proteinvetenskap

    Author : Richard Brunsell; [2021]
    Keywords : Cryo-EM; Structure determination; Protein A; Affibody; Aldolase; Scaffold; Rigid helix fusion; Photoconjugation; UV crosslinking; BPA;

    Abstract : Small proteins are difficult to study at high resolution with single-particle cryo-electron microscopy (cryo-EM). In general, sample properties such as large size (> 80 kDa), symmetry and rigidity are key to utilize this technology. READ MORE