Essays about: "transient gene expression"

Showing result 1 - 5 of 8 essays containing the words transient gene expression.

  1. 1. The role of P25 interacting transcriptional regulator VIP1 in activation of transcription.

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Asma Kanon Hashi; [2022]
    Keywords : Rhizomania; BNYVV; expression clones; virulence factor P25; transcription; transient gene expression; VIP1 plant protein;

    Abstract : Rhizomania, caused by beet necrotic yellow vein virus (BNYVV), has been considered as an economically important disease around the world because of the extreme reduction in sugar beet yield and sugar content in affected plants. The spread of rhizomania all over the world, including the emergence of resistance- breaking virus isolates, have been become a major concern for the plant pathologists and plant breeders aiming at improving sugar beet resistance to   BNYVV as well as better understanding sugar beet-virus interactions during disease development. READ MORE

  2. 2. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Gustav Myhrinder; [2020]
    Keywords : Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Abstract : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. READ MORE

  3. 3. Dissection of domain function for tandem AP2 plant transcription factors

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Harald Berg; [2018]
    Keywords : Transcription factors; plant oil biosynthesis; transient expression; Nicotiana benthamiana; domain swapping; Apetala 2; AP2; Wrinkled1; WRI1; Wrinkled4; WRI4; Baby Boom; BBM; Arabidopsis thaliana; applied biochemistry; tillämpad biokemi; Technology and Engineering;

    Abstract : Oils are some of the most energy dense compounds produced in nature and play a vital role in society as a source of food, energy and as raw material in many chemical processes. Plant produced oils (triacylglycerols) are a key asset in the transition away from fossil oils. READ MORE

  4. 4. Expression and characterization of phytoglobin genes from sugar beet (Beta vulgaris L.) in tobacco (Nicotiana benthamiana)

    University essay from SLU/Dept. of Plant Breeding (from 130101)

    Author : Axel Carl-Vilhelm Ögell Benediktsson; [2016]
    Keywords : phytoglobin; hemoglobin; transient expression; characterization; BvHb1.1; BvHb1.2;

    Abstract : Phytoglobin is a plant globin protein similar to hemoglobin, which is found in all vertebrates. Phytoglobin is categorized depending on its origin and characteristics into 6 different classes: phytoglobin class 0, phytoglobin class 1, phytoglobin class 2, symbiotic phytoglobin, legphytoglobin and phytoglobin class 3. READ MORE

  5. 5. Development and Validation of Yeast Biosensors for Signaling Pathways

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik; Lunds universitet/Fysiska institutionen

    Author : Felipe Gonzalo Tueros Farfan; [2016]
    Keywords : Xylose; yeast; signaling; Snf3 Rgt2; Snf1 Mig1; cAMP PKA; flow cytometry; high-throughput; GFP; Technology and Engineering;

    Abstract : Efficient bioconversion of the pentose xylose is vital for the development of competent lignocellulose biorefineries. Although many proper attempts of metabolic engineering for recombinant xylose utilization have been made in Saccharomyces cerevisiae, growth on xylose is still far from being optimal. READ MORE