Measurement of timescales of cortical neuronal activity in behaving mice

Abstract: Electrical activity is omnipresent throughout the brain, and it varies dependant on the brain region. Areal hierarchy has been suggested to be one of the main principles of the organization of the brain, but there is not a lot of evidence available related to the specialization of the brain’s regions in the temporal domain, that is, how the activity evolves over time. It has been suggested that there is a relationship between spatial location and timescale [1] and that the timescales of neuronal activity in rodents change according to the hierarchical position (derived from anatomical connectivity measurements) of the brain region [2]. Timescale is related to to the capability of a neuron to maintain the same firing rate over a time period. This firing rate can be measured as decay time constant of an auto-correlation matrix of spiking activity, referred to as the timescale of a single neuron [3]. In this thesis, timescales of spontaneous brain activity were measured in eight regions of the mouse prefrontal cortex (PFC) (data obtained in the Carlén Laboratory) and compared to the timescales of eight visual areas (Neuropixels Visual Coding dataset, Allen Institute for Brain Science) [4]. The results showed that cortical regions hold varying timescales, but that there is no clear correspondence of the timescales of spontaneous activity to the anatomical hierarchies. Instead, we show that the PFC regions have a greater variability in their respective timescales compared to visual cortical regions. The analysis was done using two different approaches, where for some regions the measured timescales significantly differs, due to the difference in the use of the magnitudes of the correlation. This work highlights how neuronal timescales measurements can be approached in cortical regions and used for the future work investigating their functional role and the mechanisms of generation of distinct neuronal timescales in the brain.