Discovery of peptides in Chinese medicinal plants

University essay from Uppsala universitet/Institutionen för farmaceutisk biovetenskap

Author: Mostafa Afshar; [2021]

Keywords: ;

Abstract: In this study, 20 well-known Chinese medicinal plants are included. Traditionally, these medicinal herbs were consumed for different diseases but have one application in common, treatment of rheumatism arthritis. Peptides are pharmacologically attractive substances and the reason behind this work as there is only one study on peptide content of included herbs. Commonly, plant-derived peptides are cysteine-rich peptides.  The plants were extracted in series by 60%, 30% and 10% AcN in H2O and FA. Then, the combined extracts were purified by SPE and SEC. Additionally, reduction by DDT, alkylation by IAM, and digestion by trypsin performed. UPLC-QToF was used for separation and identification of potential peptides.  The investigation resulted in finding peptides in Coix lacryma-jobi L, Atractylodes lancea, Astragalus membranaceus. Peptides found in these species are mainly in the range 2,5-5 kDa. This finding raises new questions about the function of peptides in these species and the possibility of having pharmacological activity.

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