Characterization of the equine metabolites of LGD-4033 in urine using UHPLC-MS(/MS) for doping control purposes
Abstract: The aim of this project was to study and characterize the metabolitesof LGD-4033 in equine urine, with the final aim of using the results indoping controls in equestrian sports. Urine samples had been taken atdifferent points in time from three horses that had received thesubstance intravenously. The samples were both directly analysed usinga so called dilute-and-shoot approach and were also prepared with amixed mode anion exchange solid phase extraction. All analysis weredone with UHPLC-ESI-MS(/MS) in negative mode. A total of eightmetabolites were found, which were all combinations of phase Ihydroxylation and/or phase II glucuronidation. Of these a total of four(one that is both monohydroxylated and glucuronidated, one that isdihydroxylated, as well as two glucuronidated metabolites) would besuitable for doping control.
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