Analys av haptoglobin i bovint serum med surface plasmon resonance biosensorteknik

Abstract: The concentration of acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two acute phase proteins that show the largest increase in serum concentration as a result of inflammatory processes. The haptoglobin concentration has been shown to correlate to clinical signs as well as inflammatory processes found at post mortem examination. This feature could be useful prognosis of the individual as well as for monitoring the health status of a herd or identifying carcasses that shold be examined closer at the slaughter-house. In this paper, a method for analysis of haptoglobin in milk has been modified for use with bovine sera. The method which uses optical biosensor technology is based on the haemoglobin binding properties of haptoglobin. It utilizes the interaction between analyte (haemoglobin) and biological ligand (haptogobin) which results in an optically detectable alteration in mass on the sensor surface.