Optimisation of PCR Protocol for Microsatellites in Vaccinium myrtillus : A first step in evaluating genetic diversity for future conservation

Abstract: A growing world population means an increase in crop demand. At the same time climate change threatens food security as crops may become maladapted to a new environment. We need to adapt crops to increase crop yield and become resistant to a changed environment to meet this. Crop wild relatives (CWR), which have a genetic relatedness with our crops, will be of importance as a genetic resource for crop adaption and needs to be protected. Vaccinium myrtillus is one of the prioritized species on the Nordic countries CWR priority list. Here I report PCR protocols that can be used for amplifying microsatellites, or SSRs, within V. myrtillus for use in analyses of genetic diversity within and between populations. PCRs with varying annealing temperatures (Ta) were performed. An optimal Ta for the primer pair was found for four SSR loci, and for four SSR loci, an approved Ta was found. These eight primer pairs can be used to amplify SSRs for analyses of the genetic diversity between V. myrtillus populations. This is needed to evaluate which populations to focus on in conservation programmes to ensure a broad genetic diversity usable in crop adaptation to meet increasing crop demand and climate change.