Comparison between two different freezing solutions toevaluate the sperm survival after vapor freezing andsperm preparation.

Abstract: Cryopreservation is used to freeze and store donor’s sperm, from men who are going throughcertain medical treatments such as chemotherapy or radiotherapy and from men withazoopermia or severe oligozoospermia. Stored sperm samples are used in artificialreproductive technologies. Formation of ice crystals is the biggest problem when freezingcells because of the risk severe damage to the cells. To guarantee optimal survival rates infrozen sperm samples, cryoprotectants which protect sperms from ice crystals formations, isadded.The main objective of the study was to compare two different freeze solutions and theirimpact on sperm survival in frozen sperm samples. In addition, an additional aim was todetermine if the amount of motile sperm changes when the samples is left for two hours inroom temperature.In this study, 31 samples were used. Each sample was divided into two groups. The firstgroup was mixed with a SpermFreeze Solution™ and the second group was mixed withSperm CryoProtectTM II. All samples were frozen using cooling vapor and stored in tankswith liquid nitrogen. The concentration of motile sperm was measured after thawing andpreparation. SpermFreeze SolutionTM showed significant better results in the number ofsperm who survived after freezing and thawing process Also, the results showed that werewas no significant difference on concentration of motile sperm in samples after being left atroom temperature for two hours. In summary, an improvement in the process of preparationrecommends to be performed to reduce the mechanical stress to ensure a greater quantity ofmotile sperm after the whole process.