Antibiotic free and optimised protein production using Escherichia coli

University essay from Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för biologisk grundutbildn

Abstract: Affibody® molecules are small therapeutic proteins which mimics antibody functionality. This is a report of several methods for increasing productivity and yield in recombinant production of Affibody® molecules. This literature study shows several steps in the production line which can be optimised, several novel methods for cultivating and harvesting cells and purication of proteins. There is also a section about validation of therapeutic protein production according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) are presented.

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