Modelling and simulation of antibody purification

Abstract: Due to their high specificity against target antigens, monoclonal antibodies (mAbs) have proven to be a very powerful tool for diagnostic and therapeutic purpose against several diseases. Currently there are over 26 mAb approved for therapeutic use by the FDA. The manufacture of mAbs is a complex process where purification plays a key role. This project proposes and simulates a purification process based on an array of chromatographic columns. The different employed columns were modelled by kinetic dispersive models using parameters from available bibliography as well as some assumptions. The proposed method consists of five columns connected in series and offered arguably good results with a purity and yield of the target mAb of~90% and ~85% respectively.