Active dose selection and dose-response modeling for quantitative high-throughput screening (qHTS)

Abstract: This master thesis studies the potential benefit of iterative selection of the concentrations evaluated when building mathematical dose-response curves (and response surfaces when there are two drugs) using experimental measurements. The reference alternative is to use a standard two-fold dilution series or ten-fold dilution series measured in replicates. The standard 4-parameter Hill dose-response model is used as a reference and for simulations. Models to screen for synergy between two different substances are also developed in this thesis.