Antibodies : Expression, Purification and Application
Abstract: Today antibodies are an important part in the treatment of diseases such as cancer and Guillain–Barré syndrome. It is of course important to be able to regulate the levels of the antibodies in the treatment. In this study a general method for antibody production were investigated. The antibody construct was made for transportation out of the cell. Four different tagged variants of an antibody were studied in order to find the variant best regulate by the addition of a ligand; Antibody with Tag 1, Antibody with Tag 1 and cleavage site in between, Antibody with Tag 2, and Antibody with Tag 2 and cleavage site in between. The Antibody with Tag 2 and cleavage site in between was found to be best regulated by the addition of the ligand. Despite the fact that the expression rate of this construct was not the highest, the ratio between with and without ligand gave the best dynamic range. The antibodies inside the cell weren’t regulated by the ligand and the concentration of antibodies inside the cell was higher than the concentration of antibodies outside the cell.
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