Essays about: "Hydrophobic Interaction Chromatography"

Showing result 1 - 5 of 7 essays containing the words Hydrophobic Interaction Chromatography.

  1. 1. Screening of HETP test conditions and resin storage solutions for HIC

    University essay from Uppsala universitet/Analytisk farmaceutisk kemi

    Author : Lise-Lotte Ronnerfors; [2023]
    Keywords : Hydrophobic Interaction Chromatography; Pfizer Health AB; Column packing;

    Abstract : Hydrophobic interaction chromatography (HIC) is a common purification method for biological drug substances. For continuous monitoring the quality of the purification, a column performance test is carried out to test the packing of the resin. READ MORE

  3. 2. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Keywords : Plasmid purification; pDNA; large-scale chromatography;

    Abstract : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. READ MORE

  4. 3. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    University essay from Uppsala universitet/Biokemi

    Author : Sofia Larsson; [2023]
    Keywords : ;

    Abstract : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. READ MORE

  5. 4. Unspecific interactions in different batch variations of agarose based base matrices at different NaCl concentrations.

    University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Author : Kejvan Ghofranpanah; [2022]
    Keywords : Lysozyme; hydrophobicity; size exclusion chromatography; NaCl concentrations; isocratic elution; Lysozym; hydrofobicitet; size exclusion chromatography; NaCl-koncentrationer; isokratisk eluering;

    Abstract : Size exclusion chromatography (SEC) separates molecules based on their actual size rather than their molecular weight. This indicates that the smaller a molecule is, the longer it will be held in the column since it can penetrate the pores more effectively than a bigger molecule, resulting in a longer retention time, whereas the larger molecule has a shorter retention time. READ MORE

  6. 5. Orbit-simulator for downstream processes

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Andreas Malmström; [2022]
    Keywords : Chemical engineering; Process simulation; Downstream processes; Orbit; Preparative chromatography; Python; Lumped Rate Models; Technology and Engineering;

    Abstract : In an ever more digitilized society, the transition is palpable also in the pharmaceutical industry. The control system Orbit was developed at the department of chemical engineering at Lund University, to be able to automate downstream processes based on the ÄKTA-system. READ MORE