Essays about: "Plasmid purification"

Found 4 essays containing the words Plasmid purification.

  1. 1. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Keywords : Plasmid purification; pDNA; large-scale chromatography;

    Abstract : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. READ MORE

  3. 2. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Pennie Lindblom; Olivia Geel; [2023]
    Keywords : Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Abstract : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. READ MORE

  4. 3. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elnaz Samadian Zad; [2023]
    Keywords : adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Abstract : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. READ MORE

  5. 4. Metabolic engineering of Escherichia coli for direct production of 4-hydroxybutyrate from glucose

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Sussan Alipour; [2020]
    Keywords : Escherichia coli; 4- hydroxybutyrate; 4-HB dehydrogenase; 2-oxoglutarate decarboxylase; metabolic engineering; TCA cycle; Succinyl semialdehyde;

    Abstract : Growing concerns of the negative effects on the environment and dependency of fossil fuelsare major driving forces for finding novel sustainable production pathways for plastic.Metabolic engineering has emerged as a powerful tool to enable microorganisms to producenon-native metabolites. READ MORE