Essays about: "NGS"

Showing result 21 - 25 of 43 essays containing the word NGS.

  1. 21. Characterization of co-infections and minor variants of BK polyomavirus in clinical sample by NGS

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Safia Khatoon; [2020]
    Keywords : BK polyomavirus; Co-infection; Mutation; Kidney transplant; Next generation sequencing;

    Abstract : BK polyomavirus (BKV) is associated with urinary apparatus pathogenesis in kidney transplant recipient. Immune suppression triggers BKV reactivation that potentially causes polyomavirus associated nephropathy (PVAN), a major post-transplant problem causes graft rejection. READ MORE

  3. 22. Evaluation of Oxford nanopore’s MinION : Use, functionality, and genome assembly

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : John Baxter; [2019]
    Keywords : MinION; Genome; Klebsiella; NGS;

    Abstract : The rapid and reliable detection of pathogens is of utmost importance in healthcare settings to ensure the appropriate treatment thereby reducing morbidity and mortality for the patient. Current culturing, PCR based and NGS species detection methods are time consuming (Opota et al., 2015), limited in their detection (Buckley et al. READ MORE

  4. 23. Deciphering the ontogeny of unmutated and mutated subsets of Chronic Lymphocytic Leukemia

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Ahmed Mohamed; [2019]
    Keywords : CLL; NGS; mutated CLL; unmutated CLL; CD27 bright; CD27 dull; memory B cell; RNA-sequencing;

    Abstract : Chronic Lymphocytic Leukemia (CLL) is a type of cancer that affects the B cells of the immune system causing problems in the process of producing antibodies. It can be sorted into mutated and unmutated CLL based on the percentage of somatic mutations in the Immunoglobulin Heavy chain Variable region (IgHV). READ MORE

  5. 24. Coverage Analysis in Clinical Next-Generation Sequencing

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Anna Odelgard; [2019]
    Keywords : ;

    Abstract : With the new way of sequencing by NGS new tools had to be developed to be able to work with new data formats and to handle the larger data sizes compared to the previous techniques but also to check the accuracy of the data. Coverage analysis is one important quality control for NGS data, the coverage indicates how many times each base pair has been sequenced and thus how trustworthy each base call is. READ MORE

  6. 25. Evaluation of next-generation sequencing as a tool for determining the presence of pathogens in clinical samples

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Alexander Kokkonen; [2019]
    Keywords : NGS; next generation sequencing; clinical microbiology; systems biology;

    Abstract : Metagenomic sequencing is an increasingly popular way of determining microbial diversity from environmental and clinical samples. By specifically targeting the 16S rRNA gene found in all bacteria, classifications of pathogens can be determined based on the variable and conserved regions found in the gene. READ MORE