Essays about: "molecular barcode"

Found 5 essays containing the words molecular barcode.

  1. 1. Understanding sequence errors in analysis of short tandem repeats through optimisation of a library preparation protocol utilising unique molecular indices

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Nelly Gyllö Lind; [2022]
    Keywords : polymerase chain reaction; massively parallel sequencing; DNA analysis; short tandem repeats; DNA polymerase; unique molecular indices;

    Abstract : In the last few years massively parallel sequencing (MPS) has become important for forensic DNA analysis by providing new and broader possibilities, such as increased discrimination power for short tandem repeat (STR) analyses. An imperative part of MPS analysis is library preparation where there is a fundamental reliability on in vitro DNA polymerisation, which is prone to errors. READ MORE

  3. 2. Pollen identification using sequencing techniques

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Bimaljeet Kaur; [2022]
    Keywords : pollen; rbcL gene; barcoding; metabarcoding; Sanger sequencing; MinION sequencing;

    Abstract : Palynology or the study of pollen, is essential understand the relationship between plants and their pollinators. Traditionally, pollen grains are identified by microscopy. The method has several shortcomings, such as being time-consuming and having low taxonomic resolution. READ MORE

  4. 3. Analysing Non-Desired Output Data from High Throughput Sequencers for the Identification of the Source of Contamination

    University essay from KTH/Proteinvetenskap

    Author : Mayra Guadalupe Martinez Maldonado; [2022]
    Keywords : high-throughput sequencing; metagenomics; MGI; contamination; molecular barcode; högkapacitetssekvensering; metagenomik; MGI; kontaminering; DNA-streckkod;

    Abstract : High-throughput Sequencing (HTS)-tekniker fortsätter att utvecklas snabbt, vilket ökar genomströmningen och minskar sannolikheten för fel. MGI Tech Co., Ltd. (MGI) är ett ledande HTS-varumärke som använder DNBSEQ-teknologi och finns i Center for Translational Microbiome (CTMR). READ MORE

  5. 4. Library construction optimization and analysis of Short Tandem Repeats by a simple, PCR-based DNA barcoding method

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Author : Markus André Soma; [2020]
    Keywords : Forensics; DNA; Short Tandem Repeats; Unique Molecular Identifiers; Polymerase Chain Reaction; Capillary Electrophoresis; Massively Parallel Sequencing; Applied Microbiology; Teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Abstract : DNA profiling is evolving in the forensics community towards introducing massively parallel sequencing (MPS) as a complement to capillary electrophoresis (CE). Obstacles remain however before this technology can become routine in forensic casework, such as the development of more efficient bioinformatics solutions and recommendations concerning data analyses. READ MORE

  6. 5. Molecular identification of mosquito species : Evaluation of a rapid DNA extraction method together with DNA barcoding as a tool for identification of species

    University essay from Institutionen för biologisk grundutbildning

    Author : Erik Helmersson; [2013]
    Keywords : Mosquito; DNA barcoding; PCR; sequencing; DNA; sequence; barcode; COI;

    Abstract : The current method to determine a mosquito specimen to a certain species is by morphological keys basically following the taxonomy developed by Carl Linnaeus in the 1700. Since Watson and Crick presented their model of the double-helix DNA in 1953, a new era of molecular based taxonomic studies have revolutionized the field. READ MORE