Essays about: "Enzyme engineering"

Showing result 1 - 5 of 50 essays containing the words Enzyme engineering.

  1. 1. Cloning and expressing the genes encoding Glycerol dehydratase (GDHt) and 1,3-Propanediol dehydrogenase (1,3-PDDH) in E. coli

    University essay from Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Author : Eleni Pechlivani; [2023]
    Keywords : 1; 3-Propanediol; Glycerol dehydratase; 3-Propanediol dehydrogenase; Glycerol dehydratase reactivase; E. coli; Protein expression; Biotechnology; Technology and Engineering;

    Abstract : The biodiesel sector has witnessed tremendous growth in recent years, leading to a significant increase in glycerol production as a by-product. Addressing the challenge of glycerol valorization is crucial for the sustainability of the biodiesel industry. READ MORE

  3. 2. Sortase A coupling of the recombinant partial silk proteins 4Rep-Srt and G-/G5-CT to understand the structure of silk fiber

    University essay from KTH/Proteinvetenskap

    Author : Ellen Adlerz; [2023]
    Keywords : 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A coupling; recombinant silk protein; 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A-koppling; rekombinant silkesprotein;

    Abstract : Spindelsilke är ett intressant material på grund av dess biokompatibilitet och därav användning som biomaterial. Det är jämförbart med konstgjorda material när det gäller styrka och elasticitet, och i kombination med biokompatibiliteten, är spindelsilke eftertraktat inom det medicinska området. READ MORE

  4. 3. Mechanistic Model Development for a Multi-Enzyme Protein Hydrolysis Process

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Tito Nilsson; [2023]
    Keywords : Mechanistic Model; Enzyme; Hydrolysis; Chemical Engineering; Technology and Engineering;

    Abstract : New food products are constantly put on the market. Today the focus often lies in creating nutritional and sustainable products to reduce the environmental impact of the product in question. One example of this is the approval of the yellow mealworm, Tenebrio Molitor, as a novel food in the European union. READ MORE

  5. 4. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Pennie Lindblom; Olivia Geel; [2023]
    Keywords : Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Abstract : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. READ MORE

  6. 5. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

    University essay from Linköpings universitet/Kemi

    Author : Anton Elfstrand; [2023]
    Keywords : Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

    Abstract : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. READ MORE