Essays about: "RT qPCR"

Showing result 36 - 40 of 54 essays containing the words RT qPCR.

  1. 36. Method development for sepsis biomarkers : Detection and quantification of extracted microRNA using the novel two-tailed RT-qPCR method

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Isabelle Reihell; [2020]
    Keywords : ;

    Abstract : Sepsis is a condition of the host’s extreme response to an infection affecting multiple physical functions which can lead to septic shock and death. Currently, the method for diagnosing sepsis is time-consuming and relies on multiple non-specific biomarkers such as Procalcitonin, lactate and C-reactive protein, with blood culturing being the golden standard. READ MORE

  3. 37. microRNA-200 Family Expression Level Changes in Stimulated THP-1 Cells Following NLRP3 Inflammasome Activation

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Kristina Backlund; [2020]
    Keywords : ;

    Abstract : Innate immunity is the immune systems rapid responses to infection after being attacked by a pathogen. Inflammatory responses are activated by the detection of pathogen-associated molecular patterns and danger-associated molecular patterns through pattern recognition receptors on inflammatory cells. READ MORE

  4. 38. miR-34 expression levels in relation to NLRP3 inflammasome activation in THP1-ASC-GFP cells : Pilot study

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Alyssa Smith; [2020]
    Keywords : ;

    Abstract : Inflammation is the body’s innate immune system responding to harmful stimuli. The body reacts to this stimuli by forming and activating inflammasomes. Inflammasome protein activation plays major roles seen in metabolic and autoimmune disorders, showing the importance of comprehending the processes involved. READ MORE

  5. 39. Development of bicistronic transcription and translation unit (TNT) for reliable expression of heterologous genes in Synechocystis PCC 6803

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Tereza Hubackova; [2020]
    Keywords : bicistronic design; BCD; Synechocystis PCC 6803; gene regulation; metabolic engineering;

    Abstract : Rational metabolic engineering requires tools for precise control of heterologous gene expression at predictable levels. Especially, regulatory genetic elements, such as promoters and ribosome binding sites, that can provide strong and reliable expression, are needed. READ MORE

  6. 40. Investigation of the role of insulin receptor genes in wing polyphenism using gene knockdown and differential gene expression analysis in the non-model organism Gerris buenoi

    University essay from Uppsala universitet/Institutionen för ekologi och genetik

    Author : Sofia Iggström; [2019]
    Keywords : Phenotypic plasticity; RNAi; RT-qPCR; wing polyphenism; water striders;

    Abstract : Wing polyphenism is a type of phenotypic plasticity present in several insect species whereby a genotype have the ability to develop alternative wing morphs when exposed to different environmental cues. One organism demonstrating a clear case of wing polyphenism is the water strider species, Gerris buenoi, which develop long- or short wings depending on exposure to different photoperiods (the time the organism is exposed to light during a 24 h period). READ MORE