Essays about: "HEK293"

Showing result 1 - 5 of 10 essays containing the word HEK293.

  1. 1. Development of a cell killing adenovirus with a novel cytotoxic transgene

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Carl Blomqvist; [2023]
    Keywords : ;

    Abstract : Cancer is among the most common causes of human deaths globally. Because of limitations and adverse effects of conventional cancer treatments, the need for new treatments is imminent. READ MORE

  3. 2. Compounds screening for the identification of novel drug to improve the Knock in efficiency mediated by CRISPR-Cas9

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Evangelia Anagnostou; [2023]
    Keywords : Genome editing; Knock in; HDR; CRISPR-Cas9; screening; NHEJ;

    Abstract : Genome editing is an exciting field that allows for the precise modification of an organism's DNA. One of the most advanced tools in this area is CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9), which creates a DSB (Double-strand break) at a specific location in the genome. READ MORE

  4. 3. Generation and characterization of a prostate-specific membrane antigen positive eukaryotic cell system for phage selection

    University essay from KTH/Proteinvetenskap

    Author : Linda Ehrenborg; [2021]
    Keywords : PSMA; Affibody molecules; Directed evolution; phage display; flow cytometry; PSMA; affibody; riktad evolution; fagdisplay; flödescytometri;

    Abstract : Prostate cancer is one of the most common cancer types worldwide. However, current diagnostic approaches and treatments are invasive and unspecific. Prostate-specific membrane antigen (PSMA) is an ideal biomarker for prostate cancer and can act as a target for therapeutic or diagnostic agents. READ MORE

  5. 4. Development of a high-throughput platform for generation and early screening of high producing stable cell lines

    University essay from KTH/Proteinvetenskap

    Author : Jonathan Karlsson Persson; [2021]
    Keywords : CHO-K1; HEK293; CellCelector™; High-throughput screening; Protein A beads; Secondary antibody; CHO-K1; HEK293; CellCelector™; Högkapacitets-screening; Protein A-kulor; Sekundär antikropp;

    Abstract : Produktionen av rekombinanta biofarmaceutiska läkemedel, exempelvis monoklonala antikroppar, är en oumbärlig men ansträngande process. Under 2019 var sju av de tio mest sålda läkemedlen globalt baserade på rekombinant producerade monoklonala antikroppar. READ MORE

  6. 5. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Gustav Myhrinder; [2020]
    Keywords : Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Abstract : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. READ MORE