Essays about: "Escherichia coli"

Showing result 36 - 40 of 206 essays containing the words Escherichia coli.

  1. 36. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries

    University essay from Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Nicole Eger; [2021]
    Keywords : Genotyping; Genomic barcodes; LAMP; PNA; RCA; FISH;

    Abstract : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. READ MORE

  3. 37. Production and characterisation of a chlamydial antigen candidate for vaccine trials

    University essay from Uppsala universitet/Molekylär biomimetik

    Author : Therese Koivula; [2021]
    Keywords : Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

    Abstract : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. READ MORE

  4. 38. Characterization of novel bispecific ADAPTs selected for cancer-related targets

    University essay from KTH/Proteinvetenskap

    Author : Blenda Hedin; [2021]
    Keywords : cancer therapy; alternative administration routes; ADAPT; ABD; protein technology; albumin; simultaneous bispecificity; CCL7; CEACAM5; VEGF-A;

    Abstract : Cancer is still one of the most common causes of death world-wide and in parallel there is a need to update the repertoire of therapies that withstand resistance of recurrent cancers. Since the introduction of antibody therapies as anti-cancer pharmaceuticals, recognized as immunotherapy in health care, it has been an increasing field in cancer therapy, as a more targeted treatment compared to chemotherapy. READ MORE

  5. 39. Impact of light quality on leafy green vegetables

    University essay from SLU/Dept. of Biosystems and Technology (from 130101)

    Author : Muhammad Abdul Aziz; [2021]
    Keywords : blue light; controlled light; E. coli; spinach;

    Abstract : Fruits and vegetables are a vital part of the human diet, and their consumption is on the rise because they are considered as a healthy intake. However, ingredients of fruits and vegetables are vulnerable in postharvest handling like moisture loss, wrong handling, physical damage, and microbial contamination. READ MORE

  6. 40. Investigation of possible improvements of the stability of bispecific ADAPT proteins

    University essay from KTH/Proteinvetenskap

    Author : Ella Eriksson; [2021]
    Keywords : Protein purification; Affinity chromatography; ABD-derived domain; ADAPT protein; simultan binding;

    Abstract : The aim of the project was to identify positions and amino acids that contribute to improved structure and stability of bispecific ADAPT proteins. During the 20 weeks project period, different amino acid substitutions were analysed to evaluate the effect on the three-helical structure and stability of bispecific ADAPTs targeting human serum albumin (HSA) and tumor necrosis factor α (TNFα). READ MORE