Essays about: "amber suppression"

Found 3 essays containing the words amber suppression.

  1. 1. Non-canonical amino acid incorporation as a strategy for labeling membrane bound Na+/K+-ATPase for fluorescence microscopy imaging

    University essay from KTH/Proteinvetenskap

    Author : Adam Johansson Holopainen; [2023]
    Keywords : ncAAs; fluorescence microscopy; amber suppression; nonsense suppression; Na K -ATPase; protein labeling; click chemistry; genetic code expansion; onaturliga aminosyror; fluorescensmikroskopi; Na K -ATPas; proteinmärkning; klickkemi; genetisk kodexpansion;

    Abstract : Natrium-kaliumpumpen spelar en väsentlig roll i en rad fysiologiska funktioner då den upprätthåller den elektrokemiska gradienten över cellmembranet. Ytterligare så är störningar i dess funktion associerade med flera neurologiska sjukdomar. READ MORE

  3. 2. Genetic code expansion and dCas9-DAXXHBD as synthetic routes for epigenome editing

    University essay from Högskolan i Skövde/Institutionen för hälsovetenskaper

    Author : Michael Schrempf; [2020]
    Keywords : ;

    Abstract : Epigenome editing has become a promising new tool to study the effects of singular histone modifications on the transcriptional activity of mammalian cells. Although many different approaches have been developed during the recent years many approaches used dCas9 fused epigenetic effector proteins to deposit the epigenetic mark at target sites. READ MORE

  4. 3. Improving unnatural amino acid mutagensis efficiency and selectivity in mammalian cell

    University essay from KTH/Skolan för bioteknologi (BIO)

    Author : Evan Al Saleem; [2016]
    Keywords : ;

    Abstract : Genetically encoded, site-specific incorporation of unnatural amino acids (UAA)into proteins through selective recoding of an amber stop codon provides apowerful route for expressing synthetic proteins in living cells. Recoding of theamber stop codon is achieved by introducing an amber suppressortRNA/synthetase pair orthogonal to the endogenous tRNA complement intocells. READ MORE