Essays about: "massively parallel sequencing"

Showing result 1 - 5 of 8 essays containing the words massively parallel sequencing.

  1. 1. Investigations of miR-34a structure and dynamics by SHAPE MaP and optimal time between transfection and modification by qPCR

    University essay from KTH/Proteinvetenskap

    Author : Ellinor Lindén; [2023]
    Keywords : SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a; SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a;

    Abstract : microRNA (miRNA) är korta icke-kodande RNA sekvenser som har fått ökande uppmärksamhet då de kan binda till mRNA och styra vårt genuttryck. Omkring trettio procent av våra proteinkodande gener styrs av miRNA. miRNA binder till mRNA vilket hindrar dess translation eller leder till nedbrytning av mRNA. READ MORE

  3. 2. Understanding sequence errors in analysis of short tandem repeats through optimisation of a library preparation protocol utilising unique molecular indices

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Nelly Gyllö Lind; [2022]
    Keywords : polymerase chain reaction; massively parallel sequencing; DNA analysis; short tandem repeats; DNA polymerase; unique molecular indices;

    Abstract : In the last few years massively parallel sequencing (MPS) has become important for forensic DNA analysis by providing new and broader possibilities, such as increased discrimination power for short tandem repeat (STR) analyses. An imperative part of MPS analysis is library preparation where there is a fundamental reliability on in vitro DNA polymerisation, which is prone to errors. READ MORE

  4. 3. Optimisation of ForenSeq STR data analysis with FDSTools and comparative analysis with UAS

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Tilia Thelander; [2021]
    Keywords : ;

    Abstract : DNA profiling with short tandem repeat data generated with massively parallel sequencing is associated with several challenges. FDSTools is an open-source software which applies correction models based on a reference database to correct DNA profiles. READ MORE

  5. 4. Library construction optimization and analysis of Short Tandem Repeats by a simple, PCR-based DNA barcoding method

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Author : Markus André Soma; [2020]
    Keywords : Forensics; DNA; Short Tandem Repeats; Unique Molecular Identifiers; Polymerase Chain Reaction; Capillary Electrophoresis; Massively Parallel Sequencing; Applied Microbiology; Teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Abstract : DNA profiling is evolving in the forensics community towards introducing massively parallel sequencing (MPS) as a complement to capillary electrophoresis (CE). Obstacles remain however before this technology can become routine in forensic casework, such as the development of more efficient bioinformatics solutions and recommendations concerning data analyses. READ MORE

  6. 5. Evaluation of next-generation sequencing as a tool for determining the presence of pathogens in clinical samples

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Alexander Kokkonen; [2019]
    Keywords : NGS; next generation sequencing; clinical microbiology; systems biology;

    Abstract : Metagenomic sequencing is an increasingly popular way of determining microbial diversity from environmental and clinical samples. By specifically targeting the 16S rRNA gene found in all bacteria, classifications of pathogens can be determined based on the variable and conserved regions found in the gene. READ MORE