Essays about: "spectrophotometric assay"

Showing result 6 - 8 of 8 essays containing the words spectrophotometric assay.

  1. 6. In vitro studies of Thiopurine S-Methyltransferase: Ligand binding interactions and development of a new enzymatic activity assay for TPMTwt, TPMT*6 and TPMT*8

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi; Linköpings universitet/Tekniska fakulteten

    Author : Lovisa Hemmingsson; Johan Klasén; [2015]
    Keywords : Thiopurine S-Methyltransferase; 6-mercaptourine; methotrexate; isothermal titration calorimetry; fluorescence spectroscopy;

    Abstract : Acute lymphoblastic leukemia, one of the most malignant cancer forms in children is commonly treated with the thiopurine 6-mercaptopurine (6-MP) in combination with a high dose of methotrexate (MTX). 6-Mercaptopurine is in the body metabolized by the enzyme thiopurine S-methyltransferase (TPMT). READ MORE

  3. 7. Quantitative determination of cerebrospinal fluid bilirubin on a high throughput chemistry analyzer

    University essay from Institutionen för medicinsk biokemi och mikrobiologi

    Author : Degmo Said Ahmed; [2009]
    Keywords : Bilirubin; Cerebrospinal Fluid; Diagnosis; Humans; Subarachnoid Hemorrhage;

    Abstract : Background Subarachnoid hemorrhage is a condition with high rates of mortality and morbidity. The diagnosis requires an urgent cerebral computed tomography scan and also a lumbar puncture if the scan fails to demonstrate intracranial blood. READ MORE

  4. 8. Measurement of ethanol in microdialysis samples by means of enzymatic assay using alcohol dehydrogenase and NAD

    University essay from Högskolan i Borås/Institutionen Ingenjörshögskolan

    Author : Shahin Ghoncheh; [2008]
    Keywords : alcohol dehydrogenase; ethanol; four-parameter logistic model; microdialysis; blood flow changes;

    Abstract : The enzymatic method for ethanol measurement can detect very low concentration of ethanol at samples, consequently it can’t be applied for samples with high concentration and implies as very sensitive method at limited range of detection.The alcohol dehydrogenase method is based on oxidation of alcohol in the presence of ADH as enzyme and NAD+ as coenzyme and formation of acetaldehyde and NADH that can be monitored by spectrophotometric measurement at 334,340 or 365 nm wavelengths. READ MORE