Intestinal epithelial organoid gene expression and the effects of SalmonellaTyphimurium invasion

Abstract: Salmonella species are one of the leading causes of diarrhea world-wide, and the details regarding their pathogenicity is not yet completely understood. In order to understand the interactions that occur during the invasion of the gut intestine we aimed to explore the use of intestinal epithelial organoids as a model to study the infection process of Salmonella enterica subspecies enterica serotype Typhimurium. Invasion of Salmonella Typhimurium in a two-dimensional monolayer derived from murine-derived intestinal epithelial organoids was demonstrated. Characterisation of this organoid model was attempted using real-time qPCR. In addition, this study explored the global epidemiological situation Salmonella species, with specific focus on non-typhoidal serotypes of Salmonella enterica subspecies enterica.Overall, this study shows that Salmonella species continue to be of a global concern. Intestinal epithelial organoids appear to be sensitive to experimental conditions, but ultimately serve as a good potential for in vitro studies of enteric pathogens.