Essays about: "recombinant protein expression"

Showing result 1 - 5 of 49 essays containing the words recombinant protein expression.

1. 1. Characterization of diazepam binding inhibitor as a structure-function tool for human ɣ-aminobutyric acid-A receptors

   University essay from KTH/Proteinvetenskap

   Author : Szabolcs Simon-Guth; [2023]
   Keywords : γ-Aminobutyric acid sub-type A receptors; diazepam binding inhibitor; receptor modulation; recombinant protein expression; two-electrode voltage clamp; dose-response curve; Gammaaminosmörsyrareceptor typ A; Diazepambindande hämmare; receptormodulering; reckmbinant proteinexpression; spänningsklämma med två elektroder; dosresponskurva;

   Abstract : Gammaaminosmörsyrareceptorer typ A (GABAAR) är pentameriska ligandstyrda kloridkanaler som uppvisar neurohämmande egenskaper. Därmed är de primära läkemedelsmål för flera ångestdämpande och lugnande läkemedel som används för att minska förekomsten av aktionspotential i neuroner. READ MORE

3. 2. Sortase A coupling of the recombinant partial silk proteins 4Rep-Srt and G-/G5-CT to understand the structure of silk fiber

   University essay from KTH/Proteinvetenskap

   Author : Ellen Adlerz; [2023]
   Keywords : 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A coupling; recombinant silk protein; 4RepCT; 4Rep-Srt; G- G5-CT; Sortase A-koppling; rekombinant silkesprotein;

   Abstract : Spindelsilke är ett intressant material på grund av dess biokompatibilitet och därav användning som biomaterial. Det är jämförbart med konstgjorda material när det gäller styrka och elasticitet, och i kombination med biokompatibiliteten, är spindelsilke eftertraktat inom det medicinska området. READ MORE

4. 3. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

   University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

   Author : Pennie Lindblom; Olivia Geel; [2023]
   Keywords : Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

   Abstract : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. READ MORE

5. 4. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

   University essay from Uppsala universitet/Biokemi

   Author : Milica Plavsic; [2023]
   Keywords : protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

   Abstract : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. READ MORE

6. 5. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

   University essay from Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

   Author : Erik Apelqvist; [2022]
   Keywords : Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

   Abstract : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. READ MORE