Essays about: "Nanotaggar"

Found 1 essay containing the word Nanotaggar.

  1. 1. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE