Essays about: "Plasmid transformation"

Showing result 1 - 5 of 12 essays containing the words Plasmid transformation.

  1. 1. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Wilma Wadman; [2023]
    Keywords : BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

    Abstract : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. READ MORE

  3. 2. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE

  4. 3. Evaluation of strategies for construction of protease libraries in yeast.

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Author : Sidharth Jaya Sankar; [2022]
    Keywords : Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Abstract : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. READ MORE

  5. 4. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

    University essay from Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Author : Aakash Reddy Murugadasan; [2022]
    Keywords : Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

    Abstract : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. READ MORE

  6. 5. The use of a CRISPR-Cas9 system to protect probiotic strains from transferrable drug resistance genes

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Sara Lundberg; [2021]
    Keywords : CRISPR-Cas9; antibiotic resistance; horizontal gene transfer; probiotic bacteria;

    Abstract : The discovery of antibiotics have revolutionized modern medicine, facilitating the treatment of a variety of bacterial infections, enabled surgeries otherwise impossible to perform and increased life expectancy in all countries. However, the rapid development of resistance among microorganisms and the increasing numbers of clinical outbreaks caused by multiresistant bacteria have accelerated the need for new alternatives to antibiotics. READ MORE