Essays about: "Single-molecule Imaging"
Showing result 1 - 5 of 7 essays containing the words Single-molecule Imaging.
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1. Multiple-Emitter Super-resolution Imaging using the Alternating Descent Conditional Gradient Method
University essay from Lunds universitet/Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; Lunds universitet/Institutionen för astronomi och teoretisk fysik - Genomgår omorganisationAbstract : This thesis examines the state-of-the-art 2D super-resolution technique alternating descent conditional gradient (ADCG) method's ability to accurately localize fluorophores in diffraction-limited single molecule images (SMI) and analyze the impact of pre-processing and post-processing modules on ADCG's fluorophore localization. A synthetic dataset obtained from the 2013 Grand Challenge localization microscopy and a temporally linked dataset obtained from an unpublished set of Optical DNA mapping experiments performed by Jonathan Jeffet at the NanoBioPhotonix Lab at Tel-Aviv University were initially segmented to extract their noise parameters. READ MORE
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2. Developing Automated Cell Segmentation Models Intended for MERFISH Analysis of the Cardiac Tissue by Deploying Supervised Machine Learning Algorithms
University essay from KTH/KemiAbstract : Följande studie behandlar utvecklandet av automatiserade cellsegmenteringsmodeller med avsikt att identifiera gränser mellan celler i hjärtvävnad. Syftet är att möjliggöra analys av data genererad från multiplexed error-robust in situ hybridization (MERFISH). READ MORE
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3. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag
University essay from KTH/Tillämpad fysikAbstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE
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4. Improving the temporal resolution of a microspectrometer for the study of the photophysics of enhanced green fluorescent protein
University essay from KTH/Tillämpad fysikAbstract : The use of fluorescent proteins as fluorescent markers has exploded over the last decades. In particular due to the development of advanced microscopy for live cell measurements, dynamic molecular studies down to single molecule levels and for superresolution microscopy. READ MORE
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5. DNA Denaturation Mapping in Nanochannels for Bacterial Identification
University essay from Lunds universitet/Fysiska institutionen; Lunds universitet/Fasta tillståndets fysikAbstract : In the current work, partial denaturation mapping of DNA in nanochannels is studied with the goal to use this technique as a tool for the identification of bacteria. This method has the potential to simplify and speed up the diagnosis of bacterial infections. READ MORE
