Essays about: "sfGFP"

Found 2 essays containing the word sfGFP.

  1. 1. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE

  3. 2. Engineered light controlled cell development for enhanced hydrogen production in Nostoc punctiforme ATCC 29133

    University essay from Uppsala universitet/Mikrobiell kemi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Marcel Llavero Pasquina; [2016]
    Keywords : ;

    Abstract : The aim of this thesis is to enhance heterocyst-based hydrogen production inNostoc punctiforme ATCC 29133. We envision to do so by finely regulatingthe ratio of heterocyst in order to optimize the filament energy balance. Wehereby report the development of an optogenetic synthetic switch basedon the native PcpeC promoter. READ MORE