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  1. 1. Assessment of the Fluorescent Activities of Different Fluorescent Proteins for Potential Use as Biosensors in Saccharomyces cerevisiae

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Johan Davidsson; [2020]
    Keywords : Fluorescent proteins; Saccharomyces cerevisiae; Flow cytometry; CRISPR Cas9; Biosensors; Applied microbiology; Tillämpad mikrobiologi; Technology and Engineering;

    Abstract : Development of genetically modified microorganisms such as Saccharomyces cerevisiae for use as microbial cell factories to produce fine and bulk chemicals has lately proceeded very rapidly, much thanks to new gene editing methods based on a CRISPR/Cas9 system adapted from bacteria which increases the rate of genetic engineering. However, development of methods which enable fast and simple screening of the best performing strains with specific sought-after characteristics is not advancing at the same pace. READ MORE