Physical and functional properties of chromatography media : a down-scale study

University essay from Uppsala universitet/Institutionen för biologisk grundutbildning


During down scale of column chromatography a phenomenon called wall effect appears.This supports the gel bed and gives it better flow properties. Using three different media infive different sized columns this effect was investigated regarding the physical andfunctional properties of the media. The conclusion of this study is that the effect appears incolumns below 26 mm in diameter and that the impact of the wall effect is larger the morerigid the used media is.

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