Essays about: "biacore"

Showing result 1 - 5 of 15 essays containing the word biacore.

  1. 1. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE

  3. 2. Development of biophysical methods for characterization of PROTACs

    University essay from Uppsala universitet/Biokemi

    Author : Westlin Oskar; [2021]
    Keywords : ;

    Abstract : Targeting protein with the aim of ubiquitin-labelling for degradation is a new and upand coming field of drug discovery. Proteolysis targeting chimeras (PROTACs) areheterobifunctional linker molecules that connects the target molecule to an E3ubiquitin ligase, subsequent ubiquitination of the target protein initiates the proteindegradation by the proteosome system. READ MORE

  4. 3. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Gustav Myhrinder; [2020]
    Keywords : Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Abstract : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. READ MORE

  5. 4. Interactions of the Human Recombinant Proteins JUNO and IZUMO1

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Emma Lundell; [2020]
    Keywords : male infertility; surphace plasmon resonance; protein interactions; gold nanoparticles; protein immobilization; manlig infertilitet; ytplasmonresonans; proteininteraktioner; guldnanopartiklar; proteinimmobilisering;

    Abstract : Det uppskattas att 15% av alla par världen över lider avinfertilitet. Ungefär hälften beror på manlig infertilitet och 40% av dessa fall kan ännu inte förklaras. Därmed är nuvarande metoder för att diagnostisera manlig infertilitet otillräckliga och ytterligare tekniker behövs. READ MORE

  6. 5. Development of a label-free biosensor method for the identification of sticky compounds which disturb GPCR-assays

    University essay from Institutionen för biologisk grundutbildning

    Author : Hamno Mohammed Kader; [2013]
    Keywords : Surface plasmon resonance SPR ; biosensor; Biacore; G-protein-coupled receptors GPCRs ; Low molecular weight LMW compounds; C-C chemokine receptor type 5 CCR5 ; acid-sensing ion channel 1a ASIC1a ; Thrombin; Carbonic Anhydrase II CA II ; P38α MAP kinase.;

    Abstract : It is widely known that early estimates about the binding properties of drug candidates are important in the drug discovery process. Surface plasmon resonance (SPR) biosensors have become a standard tool for characterizing interactions between a great variety of biomolecules and it offers a unique opportunity to study binding activity. READ MORE