Essays about: "crispr"

Showing result 11 - 15 of 66 essays containing the word crispr.

1. 11. Knockout of transcription factor MYB28 by CRISPR/Cas9 for reducing glucosinolate content in rapeseed (Brassica napus L.)

   University essay from SLU/Dept. of Plant Breeding (from 130101)

   Author : Anusha Harshani Bogahawatta; [2023]
   Keywords : CRISPR Cas9; Glucosinolates; ; MYB28; protoplast; ; rapeseed; ; ribonucleoprotein; ; transcription factor;

   Abstract : Rapeseed cake is a protein-rich by-product from the rapeseed oil extraction process. It contains high levels of glucosinolates (GSLs), which possess anti-nutritional functions after being hydrolyzed. Moreover, the hydrolyzed products of GSLs produce an extremely bitter taste. READ MORE

3. 12. Compounds screening for the identification of novel drug to improve the Knock in efficiency mediated by CRISPR-Cas9

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Evangelia Anagnostou; [2023]
   Keywords : Genome editing; Knock in; HDR; CRISPR-Cas9; screening; NHEJ;

   Abstract : Genome editing is an exciting field that allows for the precise modification of an organism's DNA. One of the most advanced tools in this area is CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9), which creates a DSB (Double-strand break) at a specific location in the genome. READ MORE

4. 13. Utilizing CRISPR/cas9-mediated technology to treat inherited retinal diseases : A systematic review

   University essay from Högskolan i Skövde/Institutionen för biovetenskap

   Author : Yedizza Rautavaara; [2022]
   Keywords : ;

   Abstract : Inherited retinal diseases are considered as a leading cause of vision loss in a young population. Neither a permanent cure nor long-term treatment has yet been discovered. READ MORE

5. 14. Trade-offs in CRISPR Immunity against Mobile Genetic Elements

   University essay from Uppsala universitet/Molekylär systembiologi

   Author : Johanna Cederblad; [2022]
   Keywords : CRISPR; microfluidics; fluorescent microscopy; RP4 conjugative plasmid;

   Abstract : The prokaryotic adaptive immune system CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a defense mechanism that helps to protect the prokaryotic cell from invading mobile genetic elements. This project was performed at Uppsala University and served to answer whether the expression of Cascade, which is part of the CRISPR defense system, will have a negative effect on the cell that expresses it and to also determine whether the CRISPR defense system is effective enough to stop the spread of a conjugative plasmid. READ MORE

6. 15. Evaluation of strategies for construction of protease libraries in yeast.

   University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

   Author : Sidharth Jaya Sankar; [2022]
   Keywords : Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

   Abstract : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. READ MORE