Essays about: "Fluorescence microscopy"

Showing result 6 - 10 of 91 essays containing the words Fluorescence microscopy.

  1. 6. Quantification of DNA Microballs Using Image Processing Techniques

    University essay from KTH/Industriell bioteknologi

    Author : Yosef Werede Tedros; [2023]
    Keywords : Spot detection; Deep learning; Image processing; smFISH; Gene editing; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot; Detektering av punkter; Djupinlärning; Bildbehandling; smFISH; Genredigering; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot;

    Abstract : I detta examensarbete användes olika bildbehandlingstekniker för detektion och kvantifiering av DNA-mikrobollar, mer specifikt rolling circle amplification-produkter, på mikroskopibilder. Avsikten med detta arbete var att hjälpa Countagen AB utforska pipelines för bildbehandling för sin produkt där de analyserar utfallet av genredigeringsförsök på ett billigare och snabbare sätt än dagens konventionella sekvenseringsmetoder. READ MORE

  3. 7. Cell Identification from Microscopy Images using Deep Learning on Automatically Labeled Data

    University essay from Lunds universitet/Institutionen för elektro- och informationsteknik

    Author : Fredrik Salomon-Sörensen; [2023]
    Keywords : Deep Learning; Cell Identification; Image Segmentation; Nuclei Segmentation; Convolutional Neural Networks; UNet; Image Analysis; Microscopy; Noisy Labels; Phase-Contrast; Automatic Labeling; Technology and Engineering;

    Abstract : In biology, cell counting provides a fundamental metric for live-cell experiments. Unfortunately, most researchers are constrained to using tedious and invasive methods for counting cells. Automatic identification of cells in microscopy images would therefore be a valuable tool for such researchers. READ MORE

  4. 8. Non-canonical amino acid incorporation as a strategy for labeling membrane bound Na+/K+-ATPase for fluorescence microscopy imaging

    University essay from KTH/Proteinvetenskap

    Author : Adam Johansson Holopainen; [2023]
    Keywords : ncAAs; fluorescence microscopy; amber suppression; nonsense suppression; Na K -ATPase; protein labeling; click chemistry; genetic code expansion; onaturliga aminosyror; fluorescensmikroskopi; Na K -ATPas; proteinmärkning; klickkemi; genetisk kodexpansion;

    Abstract : Natrium-kaliumpumpen spelar en väsentlig roll i en rad fysiologiska funktioner då den upprätthåller den elektrokemiska gradienten över cellmembranet. Ytterligare så är störningar i dess funktion associerade med flera neurologiska sjukdomar. READ MORE

  5. 9. Comparison and optimization of extracellular vesicle (EV) capturing on functional thin films for their molecular profiling

    University essay from KTH/Tillämpad fysik

    Author : Prattakorn Metem; [2023]
    Keywords : extracellular vesicle; plasmonic nanohole array; atomic force microscopy; fluorescence analysis; extracellulära vesiklar; nanohålsuppsättning; atomkraftsmikroskopi; fluorescensanalys;

    Abstract : Extracellular vesicles (EVs) are lipid bilayer encapsulated nanoparticles which have emerged as an excellent source of biomarkers for multiple diseases, including cancer. However, they are highly heterogeneous in their molecular compositions which remains a major challenge hindering the utilization of their biomarker potential. READ MORE

  6. 10. Co-evolutional anaylsis of the Na+,K+-ATPase’s β-subunit dimerization

    University essay from KTH/Tillämpad fysik

    Author : Sebastian Bauer; [2023]
    Keywords : Na ; K -ATPase NKA ; Co-evolution; Dimerization; Direct coupling analysis DCA ; Molecular dynamic MD simulation; Contact filtering; Mapping; Na ; K -ATPas NKA ; Samevolution; Dimerisering; Direkt kopplingsanalys DKA ; Molekylär dynamisk MD simulering; Kontaktfiltrering; Kartläggning;

    Abstract : Does the active membrane transporter, Na+,K+-ATPase dimerize? If it does, whatis the functional benefit? Does it increase or decrease the turnover rate? Theseare still unanswered questions and current research topics. Previous studies havedemonstrated dimerizations in closely related proteins of the P-type ATPase family. READ MORE