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Found 2 essays matching the above criteria.

  1. 1. The investigation of the biotransformation products formed by Cunninghamella elegans for different classes of drugs by the use of UPLC Q-TOF MS

    University essay from Uppsala universitet/Avdelningen för analytisk farmaceutisk kemi

    Author : Hanna Thorén; [2015]
    Keywords : UPLC Q-TOF MS; Glucosides; Glucuronides; TEMPO reaction; Cunninghamella elegans;

    Abstract : The fungus Cunninghamella elegans has in many studies shown to have abiotransformation similar to the metabolism of mammals. If the biotransformation isgeneral, it enables the production of metabolites by the fungus and the use asreference material. The purpose of the project were to examine whether themetabolic process of C. READ MORE

  2. 2. Rye cell wall β-glucosidase: subcloning, expression and purification of recombinant protein from E.coli

    University essay from Institutionen för livsvetenskaper

    Author : Nicolas Rochereau; [2007]
    Keywords : rye; defense system; β-glucosidase; subcloning; his-tag; thioredoxin-tag; E.coli; Ni-NTA; western blot;

    Abstract : Several plant defense systems consist of enzymes that act on glucosides and produce a toxic compound. In the intact plant tissue the substrate and enzyme are kept apart. The system studied here consists of the substrate 2-O-β-D-glucopyranosyl-4-dihydroxy-1,4-benzoxazin-3-one and the enzyme glucan 1,3-β-glucosidase in rye. READ MORE