Essays about: "flow cytometry"

Showing result 6 - 10 of 94 essays containing the words flow cytometry.

  1. 6. Platelet function after storage in leukocyte : reduced whole blood and preheating to 37°C

    University essay from Örebro universitet/Institutionen för medicinska vetenskaper

    Author : Alma Bolmsvik; Sofia Bjelkvik; [2023]
    Keywords : Blood platelets; blood transfusion; flow cytometry; blood cell count; platelet membrane glycoproteins; platelet activation.;

    Abstract : Introduction: Whole blood transfusions are indicated for the resuscitation of patients with hemorrhagic trauma. In Sweden, whole blood is stored for 14 days at 4°C. If possible, refrigerated blood is rewarmed to 37°C before transfusion to avoid hypothermia. Platelets contribute to hemostasis and can be activated with several pathways. READ MORE

  3. 7. Genetic manipulation to improve efficacy of dendric cell adoptive immunotherapy against cancer in dogs

    University essay from Karlstads universitet

    Author : Felicia Berglund; [2023]
    Keywords : Adoptive immunotherapi;

    Abstract : To improve the efficacy of the dendritic cell vaccine Alv B DC from Alv B, the PD-L1 expression in cancer cells was attempted to be reduced through a transfection with a custom designed siRNA. Before transfecting the dendritic cells, the siRNA functionality had to be tested through flow cytometry, that resulted in negative results and therefore led to a RT-qPCR protocol that indicated that the siRNA was functional. READ MORE

  4. 8. Machine learning-assisted image analysis and metabarcoding for monitoring of plankton in the seas surrounding Sweden

    University essay from KTH/Industriell bioteknologi

    Author : Karin Garefelt; [2023]
    Keywords : Imaging FlowCytobot; imaging-in-flow cytometry; plankton imaging; convolutional neural network; metabarcoding; Imaging FlowCytobot; flödesmikroskopi; övervakning av plankton; konvolutionella neuronnät; DNA-streckkodning;

    Abstract : I miljöövervakningen av haven runt Sverige har manuell mikroskopi av plankton länge varit den huvudsakliga tekniken för att övervaka växtplanktonbestånden och algblomningar. Nya tekniker utvärderas, men det är inte känt hur resultaten från de nyare teknikerna relaterar till varandra. READ MORE

  5. 9. Hypoxia and hematopoietic stem cell control with the substance Adaptaquin : An evaluation of hematopoietic stem cell’s proliferation and differentiation in artificially induced hypoxia

    University essay from Malmö universitet/Institutionen för biomedicinsk vetenskap (BMV)

    Author : Jens Christiansen; [2023]
    Keywords : Adaptaquin; Flow cytometry; Hematopoietic stem cells; Hypoxia; Hypoxia inducible factor; Prolyl hydroxylase; Adaptaquin; Flödescytometri; Hematopoetiska stamceller; Hypoxi; Hypoxi inducerande faktor; Prolylhydroxylas;

    Abstract : Hematopoietic stem cells (HSCs) have historically been difficult to maintain ex vivo with many attempts to culture them in vitro by mimicking their natural biological environment. Providing a hypoxic environment is one way to achieve this goal and can be performed by using hypoxia stimulating compounds that inhibits the degradation of HIF1a which plays an important role in regulating hypoxia. READ MORE

  6. 10. Characterisation and Identification of Human Mesenchymal Stromal Cells and the Impact of Different Culturing Media

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Sana Said Yahya; [2023]
    Keywords : Culturing media; Fetal bovine serum; Human serum; Cell proliferation; In-vitro differentiation;

    Abstract : Background: Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types and possess immunomodulatory and anti-inflammatory effects, making them interesting candidates for therapeutic applications. MSCs are present in small quantities in tissues like bone marrow and therefore need to be expanded while preserving their essential characteristics. READ MORE