Essays about: "overflow metabolism"

Found 4 essays containing the words overflow metabolism.

  1. 1. Soft sensor application on lactate controlled fed-batch cultivation for monoclonal antibody production

    University essay from Linköpings universitet/Teknisk biologi; Linköpings universitet/Tekniska högskolan

    Author : Karlberg Micael; [2015]
    Keywords : ;

    Abstract : Monoclonal antibody producing cells are of great interest and used frequently in the field of biomedical research, diagnostics and therapy with increasing need for better systems to more efficiently produce antibodies at a lower costs. In this project three fed-batch cultivations of hybridoma cells (HB-8696) were cultured in a stirred tank reactor with the use of a soft sensor to monitor the lactate concentration and as well as a dielectric probe for biomass measurements. READ MORE

  3. 2. Evaluation of on-line cell viability and L-lactate measurements in soft sensor for mammalian cell cultures

    University essay from Linköpings universitet/Teknisk biologi; Linköpings universitet/Tekniska högskolan

    Author : Alexander Reissig; [2014]
    Keywords : hybridoma; B lymphocytes; batch reactor; soft sensor; lactate; L-lactate; radio-frequency impedance; BioSenz; enzyme thermistor; overflow metabolism;

    Abstract : Increasing demand on more effective cell culture reactors has driven optimization works to increase output of products. This has led to development of soft sensors that uses mathematical formulas to increase the available information for the parameters during runs. READ MORE

  4. 3. On-line control of glucose feeding in an Escherichia coli fed-batch cultivation expressing a recombinant protein.

    University essay from Teknisk biologi

    Author : Robert Gustavsson; [2011]
    Keywords : Soft sensors; Escherichia coli; Green fluorescent protein; Overflow metabolism and mixed acid fermentation; Glucose regulation;

    Abstract : Soft sensors have been suggested as potent tools for on-line estimations of critical bioprocess variables to be able to control the biological process in an as high extent as possible. The formation of inhibitory by-products in the form of organic acids, caused by an overflow of glucose, is a problem in most bioprocesses expressing recombinant proteins. READ MORE

  5. 4. Probing Control in B. licheniformis Fermentations

    University essay from Lunds universitet/Institutionen för reglerteknik

    Author : Ola Johnsson; [2010]
    Keywords : Technology and Engineering;

    Abstract : In industrial biotechnical processes in fed-batch mode it is desirable to keep a high feed rate in order to achieve a high production rate, while simultaneously avoiding overflow metabolism. This means that the optimum of the process is close to the point where it risks crashing due to the effects of overflow metabolites. READ MORE