Active dielectrophoretic trapping for deterministic single-cell encapsulation in droplet microfluidics

Abstract: The research work focuses on optimizing various parameters for controlling cells using negative dielectrophoresis and entrapping them in droplet microfluidics. This is achieved by developing a conductivity medium, combining CytoRecovery, BSA, and EDTA, to maintain a steady count of single cells with good viability over an extended period. The study involves the optimization of frequency and voltage applied to the electrodes to achieve the desired dielectrophoretic forces for long-term cell manipulation. The optimization is based on simulations performed using myDEP and COMSOL software. Additionally, the stability of the conductivity medium is tested during prolonged electric field applications. Considering the significance of working with cells, ensuring the temperature inside the channels remains within physiological limits is vital. Both COMSOL simulations and physical experiments using Rhodamine B dye are conducted to achieve this objective. Moreover, a well-designed process flow is proposed for performing cellular entrapment in droplets. Finally, a novel microfluidic cleaning protocol has been developed to efficiently eliminate both non-biological and biological contaminants from the microfluidic chamber. This innovative protocol has the potential to enable the reuse of any microfluidic chip that does not possess a functionalized surface.