Essays about: "HILIC"

Showing result 1 - 5 of 10 essays containing the word HILIC.

1. 1. Development of a Method for Separation of Adenine Nucleotides

   University essay from Lunds universitet/Centrum för analys och syntes

   Author : Erika Miegel; [2020]
   Keywords : analytical chemistry; HPLC; HILIC; chromatography; technical analytical chemistry; teknisk analytisk kemi; Chemistry; Technology and Engineering;

   Abstract : Adenosine tetraphosphate, or Ap4A, is a nucleotide with very interesting proposed biological effects. Previous studies have indicated that blood glucose levels in mammals might increase in the presence of Ap4A, and that Ap4A might antagonize pancreatic ATP channels. READ MORE

3. 2. Optimization of a Methodology for Cell Based Untargeted Metabolomics with UHPLC-Q-ToF-MS

   University essay from Uppsala universitet/Analytisk farmaceutisk kemi

   Author : Jens Westerinen; [2020]
   Keywords : ;

   Abstract : Metabolomics is the study of small molecules (molecular weight < 1500 Da) in a biological system and can provide relevant information about endpoints of biochemical pathways by establishing metabolic profiles for different sample cohorts and comparing these through multivariate data analysis. The aim of this thesis was to establish and test a suitable methodology for cell-based untargeted metabolomics, utilizing UHPLC-MS. READ MORE

4. 3. Development of UPLC-MS/MS method for the determination of polar metabolites

   University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

   Author : Gustav Norin; [2018]
   Keywords : UPLC-MS MS; HILIC; TMAO; betaine; method development;

   Abstract : Trimethylamine-n-oxide (TMAO) is a metabolite found in plasma/serum in humans. Elevated levels of TMAO have been associated with several types of heart disease. It’s therefore of interest to make a simple analytical method to analyse TMAO and other metabolites that are degraded to TMAO, including betaine. READ MORE

5. 4. Conditioning of chromatographic systems prior to metabolomic studies : Investigation of the conditioning effect and the possibility to alter it

   University essay from Uppsala universitet/Avdelningen för analytisk farmaceutisk kemi

   Author : Jasmin Telo; [2017]
   Keywords : Analytical chemistry; metabolomics; metabonomics; conditioning; conditioning effect; UPLC; Q-TOF; HILIC; Protein precipitation; Liquid-liquid extraction;

   Abstract : The conditioning effect in metabolomic studies is the phenomenon of initial variation of analytical results in the first 5-10 injections of a biological sample in chromatographic systems. The deviation manifests itself as a drift in retention time, peak area and in multivariate analysis. READ MORE

6. 5. Photodegradation study of 3,5-diamino-6-chloro- N-(2-(methylamino)ethyl)pyrazine-2-carboxamide using preparative SFC and LC-MS

   University essay from Uppsala universitet/Institutionen för farmaci

   Author : Sara Sillén; [2016]
   Keywords : photodegradation; UPLC; SFC; liquid chromatography; supercritical fluid chromatography; mass spectrometry; freeze drying; 18O-water; analytical chemistry; structure elucidation; photodegradants;

   Abstract : In this project the photodegradation of 3,5-diamino-6-chloro-N-(2-(methylamino)ethyl)pyrazine-2-carboxamide was studied. A hypothetical degradation pattern for the compound was proposed and the aim of the project was to study the formed secondary photodegradants and to, if possible, structure elucidate some of these compounds. READ MORE