Essays about: "IgG production"

Showing result 1 - 5 of 17 essays containing the words IgG production.

  1. 1. Development of an AVI-tagged phagemid vector

    University essay from KTH/Proteinvetenskap

    Author : Barathram Swaminathan; [2022]
    Keywords : phage display; affibody; directed evolution; protein engineering; protein interactions; fagdisplay; affibody; riktad evolution; protein engineering; proteininteraktioner;

    Abstract : Fagmider är ett kraftfullt verktyg som använts för att uttrycka heterologa proteiner på fagvirus i metoder som exempelvis riktad evolution via fagdisplay av proteinbinliotek. Monovalent uttryck genom fagemider lider av ett överflöd av kala fager som är svåra att rensa bort. READ MORE

  3. 2. Design, production and evaluation of cross linked target proteins to an affibody-based carrier framework aimed for affinity protein: antigen structure determination using single particle Cryo-EM

    University essay from KTH/Proteinvetenskap

    Author : Richard Brunsell; [2021]
    Keywords : Cryo-EM; Structure determination; Protein A; Affibody; Aldolase; Scaffold; Rigid helix fusion; Photoconjugation; UV crosslinking; BPA;

    Abstract : Small proteins are difficult to study at high resolution with single-particle cryo-electron microscopy (cryo-EM). In general, sample properties such as large size (> 80 kDa), symmetry and rigidity are key to utilize this technology. READ MORE

  4. 3. Class switching mechanism in antibodies : A conformational and structural investigation of the B-cell repertoire diversity of the CDR3

    University essay from Högskolan i Skövde/Institutionen för hälsovetenskaper

    Author : Sofia Fredin; [2021]
    Keywords : ;

    Abstract : A process called class switching recombination (CSR) regulates the production of isotypes by enabling the switch of producing unspecific IgM or IgD to producing IgG, IgE, or IgA by causing an irreversible genomic rearrangement. Recent studies indicate a gap in the identification of the molecular mechanisms underlying CSR. READ MORE

  5. 4. Production and validation of anti-HCV antibodies for viral neutralization

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Megan Olga García Mark; [2020]
    Keywords : Cloning; antibody; infection; HCV; IgG production; molecular biology; cell factory; plasmid design; immunology;

    Abstract : Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. READ MORE

  6. 5. Selection of a calcium-dependent IgG1-binding protein domain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Sanne Rönning; [2020]
    Keywords : protein science; phage display; selection; Protein A; chromatography; calcium dependence; characterization; proteinteknik; fagdisplay; selektion; Protein A; kromatografi; calciumberoende; karakterisering;

    Abstract : Standard purification processes in large scale antibody production are largely dependent on Protein A chromatography. Protein A binds specifically to many subclasses of IgG with high affinity. However, in order to elute the proteins, the pH needs to be lowered. READ MORE