Essays about: "Next-generation sequencing"

Showing result 1 - 5 of 52 essays containing the words Next-generation sequencing.

  1. 1. Validation of a Next Generation Sequencing based method for chimerism analysis in clinical practice

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Maria Högberg; [2022]
    Keywords : Devyser chimerism; fragment analysis; hematopoietic stem cell transplantation; short tandem repeats; indels;

    Abstract : Hematopoietic stem cell transplantation (HSCT) is used to treat patient with hematological diseases such as leukemia and genetic conditions such as sickle cell anemia. After HSCT the patients are supervised for signs of relapse of disease or rejection of transplanted cells. This is done by using chimerism analysis. READ MORE

  2. 2. Filtering of Clinical NGS Data to Improve Low Allele Frequency Variant Calling

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Tomas Cumlin; [2022]
    Keywords : Next-generation sequencing; Variant calling; Clinical sequencing; Substitution error; Cancer sequencing; Error rate; substitution; Somatic mutation;

    Abstract : Massive parallel sequencing (NGS) is useful in detecting and later classifying somatic driver mutations in cancer tumours. False-positive variants occur in the NGS workflow and they may be mistaken for low frequency somatic cancer mutations in a patient sample. READ MORE

  3. 3. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE

  4. 4. Sepsis-associated Escherichia coli whole-genome sequencing analysis using in-house developed pipeline and 1928 diagnostics tool

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Geivi Lember; [2021]
    Keywords : ;

    Abstract : Sepsis is a life-threatening condition that is caused by a dysregulated host response to infection. Timely detection of sepsis and antibiotic treatment is important for the patient’s recovery from sepsis. READ MORE

  5. 5. Modelling of the DNA Helix’s Duration for Genome Sequencing

    University essay from KTH/Skolan för elektroteknik och datavetenskap (EECS)

    Author : Sabina Dzubur; Rim Sharif; [2021]
    Keywords : Nanopore sequencing; DNA sequencing; Linear regression; Supervised learning; Distribution fit;

    Abstract : Nanopore sequencing is the next generation ofsequencing methods which promises to deliver cheaper andmore portable genome sequencing capabilities. A single DNAor RNA strand is passed through a nanopore nested in anartificial membrane with an electric potential applied across it. READ MORE