Essays about: "Next-generation sequencing"

Showing result 11 - 15 of 66 essays containing the words Next-generation sequencing.

  1. 11. Evaluation and implementation of quality control parameters for genome-wide DNA methylome sequencing

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Sara Ekberg; [2022]
    Keywords : ;

    Abstract : Epigenomics is the study of modifications to the genetic material without changes to the DNA sequence, one such modification is methylation of nucleotides. DNA methylation is associated with gene regulation and is studied in a variety of fields such as cancer and ageing. READ MORE

  3. 12. Filtering of Clinical NGS Data to Improve Low Allele Frequency Variant Calling

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Tomas Cumlin; [2022]
    Keywords : Next-generation sequencing; Variant calling; Clinical sequencing; Substitution error; Cancer sequencing; Error rate; substitution; Somatic mutation;

    Abstract : Massive parallel sequencing (NGS) is useful in detecting and later classifying somatic driver mutations in cancer tumours. False-positive variants occur in the NGS workflow and they may be mistaken for low frequency somatic cancer mutations in a patient sample. READ MORE

  4. 13. Developing a Method to Study the Impact of Microbial DNA on the Brain Related to Alzheimer’s Disease – Can Food Make a Change?

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master)

    Author : Fuh-Tzu Yang; [2022]
    Keywords : DNA extraction; Microbiome DNA enrichment; Host DNA depletion; 16S rRNA sequencing; Alzheimer’s disease; Gut-brain axis; Microbiota; Microbial DNA; Black currant; Applied Nutrition and Food Chemistry; Medicine and Health Sciences;

    Abstract : Understanding the gut-brain-microbiota axis can help us provide a new tool to manage conditions such as Alzheimer’s. However, a major challenge is selectively amplifying the bacterial DNA which is present at a much lower level than the host DNA. READ MORE

  5. 14. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE

  6. 15. Implementing ExomeDepth with a variant filter as a CNV-calling tool for germline clinical diagnostic testing

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Alice Krysén; [2022]
    Keywords : ;

    Abstract : Copy number variations (CNVs) cover approximately 4.9 - 9.5% of the human genome. CNVs are involved in both the development of disease and evolutionary adaptions. READ MORE