Essays about: "Protein Crystallization"

Showing result 16 - 20 of 27 essays containing the words Protein Crystallization.

  1. 16. Single-molecule X-ray free-electron laser imaging : Interconnecting sample orientation with explosion data

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Christofer Östlin; [2014]
    Keywords : X-ray; free-electron laser; XFEL; diffraction analysis; structure determination; nanocrystal; molecular dynamics; GROMACS; biomolecular imaging; ubiquitin; trajectory; explosion;

    Abstract : X-ray crystallography has been around for 100 years and remains the preferred technique for solving molecular structures today. However, its reliance on the production of sufficiently large crystals is limiting, considering that crystallization cannot be achieved for a vast range of biomolecules. READ MORE

  3. 17. Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Roland Bergdahl; [2013]
    Keywords : Biogas; Syntrophic acetate oxidizing bacteria; Tepidanaerobacter acetatoxydans Re1; FTHFS; N10- formyltetrahydrofolate synthetase; Crystallography; Structure determination;

    Abstract : The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolution, respectively. READ MORE

  4. 18. Expression, purification and characterization of Violaxanthin De-epoxidase from Spinach

    University essay from Lunds universitet/Kemiska institutionen

    Author : Erik Hallin; [2011]
    Keywords : Proteinvetenskap; Chemistry;

    Abstract : Plants require sunlight to be able to perform photosynthesis but an excess of intense sunlight is harmful to the organism and therefore needs a method to protect itself. Violaxanthin De-Epoxidase (VDE) is located inside the thylakoid lumen and catalyzes the de-epoxidation of violaxanthin to zeaxanthin. READ MORE

  5. 19. Purification and crystallization of the I subunit of magnesium chelatase

    University essay from Lunds universitet/Kemiska institutionen

    Author : You Lv; [2011]
    Keywords : Magnesium chelatase; BchI; ChlI; AAA protein; Proteinvetenskap; Chemistry;

    Abstract : The I subunit of magnesium chelatase is an AAA+ protein which binds and hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. READ MORE

  6. 20. Structure-function study on the activation mechanism of Endo T : Crystallization and biochemical characterization of the intact Endo T from Trichoderma reesei (Hypocrea jecorina)

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Jonathan Boel; [2011]
    Keywords : Hypocrea jecorina Endo T; glycoside hydrolase family 18; EC 3.2.1.96; deglycosylation; proteolysis; C-terminus; protein structure;

    Abstract : Endo T is a deglycosylating enzyme secreted by the filamentous fungus Trichoderma reesei (Hypocrea jecorina). The active Endo T protein is both deglycosylated and C-terminal processed. Since the intact protein was never observed in the T. reesei culture medium, a construct of the intact Endo T was expressed in P. READ MORE